Description

A position for a PostDoc is available immediately in the Cowburn lab at Albert Einstein College of Medicine, focussed on NMR based structural biology.  Areas of focus are characterization of interactions of intrinsically disordered systems in selective transport in the nuclear pore  (Hough et al., 2015; Raveh et al., 2016; Sparks et al., 2020; Sparks et al., 2018) and protein engineering applications and basic mechanisms of inteins (Eryilmaz et al., 2014; Stevens et al., 2018; Stevens et al., 2017)   In addition the these focal areas, the lab has extensive NMR related collaborations e.g. (Dixit et al., 2021; Warren et al., 2017) .

 The successful candidate will benefit from access to outstanding resources in NMR and X-ray crystallography including a SampleJet auto sampler, robotic dispensers for NMR sample preparation and crystallization, protein production facilities (E. coli, baculovirus and mammalian cells), biophysical analysis (MST, FP, ITC, SPR, DLS), proteomics, plus Linux clusters and use of XSEDE for computational modeling and calculations. Our laboratory has access to high-field NMR including two modern in house 600 MHz NMR spectrometers, two 900 MHz and three 800 MHz spectrometers at the New York Structural Biology Center (NYSBC). NYSBC also provides access to additional resources in X-ray crystallography, and Cryo-electron microscopy.  Resources at National Labs are available for SAXS and SANS. 

Dixit, U., Bhutoria, S., Wu, X., Qiu, L., Spira, M., Mathew, S., Harris, R., Adams, L.J., Cahill, S., Pathak, R., et al. (2021). INI1/SMARCB1 Rpt1 domain mimics TAR RNA in binding to integrase to facilitate HIV-1 replication. Nat Commun 12, 2743.
Eryilmaz, E., Shah, N.H., Muir, T.W., and Cowburn, D. (2014). Structural and dynamical features of inteins and implications on protein splicing. J Biol Chem 289, 14506-14511.
Hough, L.E., Dutta, K., Sparks, S., Temel, D.B., Kamal, A., Tetenbaum-Novatt, J., Rout, M.P., and Cowburn, D. (2015). The molecular mechanism of nuclear transport revealed by atomic-scale measurements. Elife 4.
Raveh, B., Karp, J.M., Sparks, S., Dutta, K., Rout, M.P., Sali, A., and Cowburn, D. (2016). Slide-and-exchange mechanism for rapid and selective transport through the nuclear pore complex. Proc Natl Acad Sci U S A 113, E2489-2497.
Sparks, S., Hayama, R., Rout, M.P., and Cowburn, D. (2020). Analysis of Multivalent IDP Interactions: Stoichiometry, Affinity, and Local Concentration Effect Measurements. Methods in molecular biology (Clifton, NJ 2141, 463-475.
Sparks, S., Temel, D.B., Rout, M.P., and Cowburn, D. (2018). Deciphering the "Fuzzy" Interaction of FG Nucleoporins and Transport Factors Using Small-Angle Neutron Scattering. Structure 26, 477-484 e474.
Stevens, A.J., Sekar, G., Gramespacher, J.A., Cowburn, D., and Muir, T.W. (2018). An Atypical Mechanism of Split Intein Molecular Recognition and Folding. J Am Chem Soc 140, 11791-11799.
Stevens, A.J., Sekar, G., Shah, N.H., Mostafavi, A.Z., Cowburn, D., and Muir, T.W. (2017). A promiscuous split intein with expanded protein engineering applications. Proc Natl Acad Sci U S A 114, 8538-8543.
Warren, C., Matsui, T., Karp, J.M., Onikubo, T., Cahill, S., Brenowitz, M., Cowburn, D., Girvin, M., and Shechter, D. (2017). Dynamic intramolecular regulation of the histone chaperone nucleoplasmin controls histone binding and release. Nat Commun 8, 2215.

Requirements

The candidate will have a Ph.D. in a relevant area with a  record demonstrating experience in NMR data acquisition and analysis, and protein-protein/ligand interactions. Additional experience in structural biology, biochemistry, biophysics, chemical biology and protein expression are advantages.   The salary and benefits for this position, including career development opportunities, are highly competitive.

 Applicants should send a cover letter, including 1-2-page summary of past and present research interests and accomplishments, and a curric­­ulum vitae with names and contact details of three references.